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In vitro Crystallization of Ribosomes from Chick Embryos

R. A. Milligan and P. N. T. Unwin
The Journal of Cell Biology
Vol. 95, No. 2, Part 1 (Nov., 1982), pp. 648-653
Stable URL: http://www.jstor.org/stable/1610137
Page Count: 6
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In vitro Crystallization of Ribosomes from Chick Embryos
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Abstract

A new two-dimensional ribosome crystal, having the tetragonal space group P4212 (a = 593 Å), has been grown from ribosome tetramers extracted from hypothermic chick embryos. It is of particular interest because of its larger size (up to 3× 3μ m2) and greater stability compared to other related polymorphic forms, and because it can easily be grown in large amounts. X-ray diffraction shows the order in the crystal to extend to a resolution of at least 60 Å. The crystalline ribosomes appear to contain a full complement of small and large ribosomal subunit proteins and an additional four proteins not characteristic of chick embryo polysomes.

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