Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Growth-Inhibitory Activity of Lymphoid Cell Plasma Membranes. I. Inhibition of Lymphocyte and Lymphoid Tumor Cell Growth

Kathryn C. Stallcup, Andrea Dawson and Matthew F. Mescher
The Journal of Cell Biology
Vol. 99, No. 4, Part 1 (Oct., 1984), pp. 1221-1226
Stable URL: http://www.jstor.org/stable/1611047
Page Count: 6
  • More info
  • Cite this Item
Growth-Inhibitory Activity of Lymphoid Cell Plasma Membranes. I. Inhibition of Lymphocyte and Lymphoid Tumor Cell Growth
Preview not available

Abstract

Membranes isolated from normal spleen cells or lymphoid tumor cells were found to inhibit in vitro growth of several murine tumor cell lines including a B cell hybridoma, a thymoma, and a mastocytoma. 50% inhibition occurred at membrane protein concentrations of 60-100 μg/ml. A similar concentration dependence was found for inhibition of [3H]-thymidine incorporation by tumor cells and for the lipopolysaccharide-induced mitogenic response of normal spleen cells. The inhibitory activity co-purified with the plasma membrane upon fractionation of crude membranes. Membrane solubilization with deoxycholate followed by dialysis to remove the detergent gave good recovery of inhibitory activity in the resulting reconstituted membranes. Membrane-mediated growth inhibition resulted from a decreased rate of proliferation and not from increased cell death. A toxic effect of the membranes was further ruled out by the finding that increasing the fetal calf serum content of the medium could substantially reverse the growth inhibition. Thus, the plasma membrane of lymphoid cells contains a component that can slow or stop the growth of cells in culture. This membrane component may have a role in cell contact-mediated regulation of growth.

Page Thumbnails

  • Thumbnail: Page 
1221
    1221
  • Thumbnail: Page 
1222
    1222
  • Thumbnail: Page 
1223
    1223
  • Thumbnail: Page 
1224
    1224
  • Thumbnail: Page 
1225
    1225
  • Thumbnail: Page 
1226
    1226