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Wheat Germ Agglutinin Blocks the Acrosome Reaction in Strongylocentrotus purpuratus Sperm by Binding a 210,000-mol-wt Membrane Protein

Sheila B. Podell and Victor D. Vacquier
The Journal of Cell Biology
Vol. 99, No. 5 (Nov., 1984), pp. 1598-1604
Stable URL: http://www.jstor.org/stable/1611093
Page Count: 7
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Wheat Germ Agglutinin Blocks the Acrosome Reaction in Strongylocentrotus purpuratus Sperm by Binding a 210,000-mol-wt Membrane Protein
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Abstract

Wheat germ agglutinin (WGA) binds to the entire surface of Strongylocentrotus purpuratus sperm, and inhibits the egg jelly-induced acrosome reaction. The binding was found to be species dependent and was completely inhibited by 5 mM N-acetyl-D-glucosamine. Blockage of the acrosome reaction by WGA was bypassed by a combination of the ionophores A23187 and monensin, although neither ionophore was effective individually. These experiments suggest that WGA blocks both Ca2+ uptake and Na+/H+ exchange in these sperm, which was confirmed by direct measurements of 45Ca2+ uptake and H+ efflux. The target of WGA in S. purpuratus sperm appears to be a membrane glycoprotein of Mr=210,000. Treatment of this protein with neuraminidase or endo-β-N-acetylglucosaminidase F abolished WGA binding.

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