Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Formation of the Apical Pole of Epithelial (Madin-Darby Canine Kidney) Cells: Polarity of an Apical Protein Is Independent of Tight Junctions While Segregation of a Basolateral Marker Requires Cell-Cell Interactions

Dora E. Vega-Salas, Pedro J. I. Salas, Doris Gundersen and Enrique Rodriguez-Boulan
The Journal of Cell Biology
Vol. 104, No. 4 (Apr., 1987), pp. 905-916
Stable URL: http://www.jstor.org/stable/1612363
Page Count: 12
  • More info
  • Cite this Item
Formation of the Apical Pole of Epithelial (Madin-Darby Canine Kidney) Cells: Polarity of an Apical Protein Is Independent of Tight Junctions While Segregation of a Basolateral Marker Requires Cell-Cell Interactions
Preview not available

Abstract

The time course of development of polarity of an apical (184-kD) and a basolateral (63-kD) plasma membrane protein of Madin-Darby canine kidney cells was followed using semiquantitative immunofluorescence on semithin (∼0.5-μm) frozen sections and monoclonal antibody probes. The 184-kD protein became highly polarized to the apical pole within the initial 24 h both in normal medium and in 1-5 μM Ca2+, which results in well-spread, dome-shaped cells, lacking tight junctions and other lateral membrane interactions. In contrast, the basolateral 63-kD membrane protein developed full polarity only after incubation in normal Ca2+ concentrations for >72 h, a time much longer than that required for the formation of tight junctions (∼18 h) and failed to polarize in 1-5 μM Ca2+. These results demonstrate that intradomain restriction mechanisms independent of tight junctions, such as self-aggregation or specific interactions with the submembrane cytoskeleton, participate in the regionalization of at least some epithelial plasma membrane proteins. The full operation of these mechanisms depends on the presence of normal cell-cell interactions in the case of the basolateral 63-kD antigen but not in the case of the apical 184-kD protein.

Page Thumbnails

  • Thumbnail: Page 
905
    905
  • Thumbnail: Page 
906
    906
  • Thumbnail: Page 
907
    907
  • Thumbnail: Page 
908
    908
  • Thumbnail: Page 
909
    909
  • Thumbnail: Page 
910
    910
  • Thumbnail: Page 
911
    911
  • Thumbnail: Page 
912
    912
  • Thumbnail: Page 
913
    913
  • Thumbnail: Page 
914
    914
  • Thumbnail: Page 
915
    915
  • Thumbnail: Page 
916
    916