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Import of the Carboxy-Terminal Portion of Acyl-CoA Oxidase into Peroxisomes of Candida tropicalis

Gillian M. Small and Paul B. Lazarow
The Journal of Cell Biology
Vol. 105, No. 1 (Jul., 1987), pp. 247-250
Stable URL: http://www.jstor.org/stable/1612538
Page Count: 4
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Import of the Carboxy-Terminal Portion of Acyl-CoA Oxidase into Peroxisomes of Candida tropicalis
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Abstract

We report the sequence of a cDNA clone that codes for the carboxy-terminal portion of the peroxisomal protein, acyl-CoA oxidase, from the yeast, Candida tropicalis. This is a newly identified acyl-CoA oxidase sequence, most likely a second allele of POX4. The cDNA clone was expressed by in vitro transcription followed by translation. The major product, a 43-kD protein, associated with isolated peroxisomes in an in vitro import assay. More than half of the peroxisome-associated protein was protected from added protease, implying that it was internalized within the organelle. These findings indicate that there is sufficient information in the carboxy-terminal portion of the protein to target it to peroxisomes.

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