Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Evolutionary Conservation of Tissue-Specific Lymphocyte-Endothelial Cell Recognition Mechanisms Involved in Lymphocyte Homing

Nora W. Wu, Sirpa Jalkanen, Philip R. Streeter and Eugene C. Butcher
The Journal of Cell Biology
Vol. 107, No. 5 (Nov., 1988), pp. 1845-1851
Stable URL: http://www.jstor.org/stable/1613238
Page Count: 7
  • More info
  • Cite this Item
Evolutionary Conservation of Tissue-Specific Lymphocyte-Endothelial Cell Recognition Mechanisms Involved in Lymphocyte Homing
Preview not available

Abstract

Tissue-specific interactions with specialized high endothelial venules (HEV) direct the homing of lymphocytes from the blood into peripheral lymph nodes, mucosal lymphoid organs, and tissue sites of chronic inflammation. These interactions have been demonstrated in all mammalian species examined and thus appear highly conserved. To assess the degree of evolutionary divergence in lymphocyte-HEV recognition mechanisms, we have studied the ability of lymphocytes to interact with HEV across species barriers. By using an in vitro assay of lymphocyte binding to HEV in frozen sections of lymphoid tissues, we confirm that mouse, guinea pig, and human lymphocytes bind to xenogeneic as well as homologous HEV. In addition, we show that mouse and human lymphoid cell lines that bind selectively to either peripheral lymph node or mucosal vessels (Peyer's patches, appendix) in homologous lymphoid tissues exhibit the same organ specificity in binding to xenogeneic HEV. Furthermore, monoclonal antibodies that recognize lymphocyte "homing receptors" and block homologous lymphocyte binding to peripheral lymph node or to mucosal HEV, also inhibit lymphocyte interactions with xenogeneic HEV in a tissue-specific fashion. Similarly, anti-HEV antibodies against organ-specific mouse high endothelial cell "addressins" involved in lymphocyte homing to peripheral lymph node or mucosal lymphoid organs, not only block the adhesion of mouse lymphocytes but also of human lymphocytes to target mouse HEV. The results illustrate a remarkable degree of functional conservation of elements mediating these cell-cell recognition events involved in organ-specific lymphocyte homing.

Page Thumbnails

  • Thumbnail: Page 
1845
    1845
  • Thumbnail: Page 
1846
    1846
  • Thumbnail: Page 
1847
    1847
  • Thumbnail: Page 
1848
    1848
  • Thumbnail: Page 
1849
    1849
  • Thumbnail: Page 
1850
    1850
  • Thumbnail: Page 
1851
    1851