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Subunit Selectivity and Epitope Characterization of mAbs Directed against the GABA A/Benzodiazepine Receptor

Markus Ewert, Brenda D. Shivers, Hartmut Lüddens, Hanns Möhler and Peter H. Seeburg
The Journal of Cell Biology
Vol. 110, No. 6 (Jun., 1990), pp. 2043-2048
Stable URL: http://www.jstor.org/stable/1614088
Page Count: 6
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Subunit Selectivity and Epitope Characterization of mAbs Directed against the  GABA A/Benzodiazepine Receptor
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Abstract

mAbs bd 17, bd 24, and bd 28 raised against bovine cerebral γ-aminobutyric acid ( GABA A)/benzodiazepine receptors were analyzed for their ability to detect each of 12 GABA A receptor subunits expressed in cultured mammalian cells. Results showed that mAb bd 17 recognizes epitopes on both β 2 and β 3 subunits while mAb bd 24 is selective for the α 1 subunit of human and bovine, but not of rat origin. The latter antibody reacts with the rat α 1 subunit carrying an engineered Leu at position four, documenting the first epitope mapping of a GABA A receptor subunit-specific mAb. In contrast to mAbs bd 17 and bd 24, mAb bd 28 reacts with all GABA A receptor subunits tested but not with a glycine receptor subunit, suggesting the presence of shared epitopes on subunits of GABA-gated chloride channels.

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