Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Human fur Gene Encodes a Yeast KEX2-like Endoprotease That Cleaves Pro-β-NGF in Vivo

Patricia A. Bresnahan, Richard Leduc, Laurel Thomas, Jeremy Thorner, Helen L. Gibson, Anthony J. Brake, Philip J. Barr and Gary Thomas
The Journal of Cell Biology
Vol. 111, No. 6, Part 2 (Dec., 1990), pp. 2851-2859
Stable URL: http://www.jstor.org/stable/1614290
Page Count: 9
  • More info
  • Cite this Item
Human fur Gene Encodes a Yeast KEX2-like Endoprotease That Cleaves Pro-β-NGF in Vivo
Preview not available

Abstract

Extracts from BSC-40 cells infected with vaccinia recombinants expressing either the yeast KEX2 prohormone endoprotease or a human structural homologue (fur gene product) contained an elevated level of a membrane-associated endoproteolytic activity that could cleave at pairs of basic amino acids (-LysArg- and -ArgArg-). The fur-directed activity (furin) shared many properties with Kex2p including activity at pH 7.3 and a requirement for calcium. By using antifurin antibodies, immunoblot analysis detected two furin translation products (90 and 96 kD), while immunofluorescence indicated localization to the Golgi apparatus. Coexpression of either Kex2p or furin with the mouse β-nerve growth factor precursor (pro-β-NGF) resulted in greatly enhanced conversion of the precursor to mature nerve growth factor. Thus, the sequence homology shared by furin and the yeast KEX2 prohormone processing enzyme is reflected by significant functional homology both in vitro and in vivo.

Page Thumbnails

  • Thumbnail: Page 
2851
    2851
  • Thumbnail: Page 
2852
    2852
  • Thumbnail: Page 
2853
    2853
  • Thumbnail: Page 
2854
    2854
  • Thumbnail: Page 
2855
    2855
  • Thumbnail: Page 
2856
    2856
  • Thumbnail: Page 
2857
    2857
  • Thumbnail: Page 
2858
    2858
  • Thumbnail: Page 
2859
    2859