Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Two Forms of Elongation Factor 1α (EF-1α O and 42Sp50), Present in Oocytes, but Absent in Somatic Cells of Xenopus laevis

Stéphane Deschamps, Julia Morales, André Mazabraud, Marc le Maire, Herman Denis and Donald D. Brown
The Journal of Cell Biology
Vol. 114, No. 6 (Sep., 1991), pp. 1109-1111
Stable URL: http://www.jstor.org/stable/1614755
Page Count: 3
  • More info
  • Cite this Item
Two Forms of Elongation Factor 1α (EF-1α O and 42Sp50), Present in Oocytes, but Absent in Somatic Cells of Xenopus laevis
Preview not available

Abstract

We have purified and partially sequenced the EF-1α protein from Xenopus laevis oocytes (EF-1αO). We show that the two cDNA clones isolated by Coppard et al. do not encode 42Sp50, as claimed by these authors, but two very similar forms of EF-1αO (EF-1αO and EF-1αO1). 42Sp50 is the major protein component of a 42S nucleoprotein particle that is very abundant in previtellogenic oocytes of X. laevis. 42Sp50 differs from EF-1αO not only by its amino acid sequence, but also by several properties already reported. In particular, 42Sp50 has a low EF-1α activity. It is distributed uniformly in the cytoplasm of previtellogenic oocytes, in contrast to EF-1αO which is concentrated in a small region of the cytoplasm, known as the mitochondrial mass or Balbiani body.

Page Thumbnails

  • Thumbnail: Page 
1109
    1109
  • Thumbnail: Page 
1110
    1110
  • Thumbnail: Page 
1111
    1111