Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

An Ankyrin-Related Gene (unc-44) Is Necessary for Proper Axonal Guidance in Caenorhabditis elegans

Anthony J. Otsuka, Rodrigo Franco, Bin Yang, Kyu-Hwan Shim, Lan Zhao Tang, Yu Yong Zhang, Pratumtip Boontrakulpoontawee, Ayyamperumal Jeyaprakash, Edward Hedgecock, Virginia I. Wheaton and Alan Sobery
The Journal of Cell Biology
Vol. 129, No. 4 (May, 1995), pp. 1081-1092
Stable URL: http://www.jstor.org/stable/1616855
Page Count: 12
  • More info
  • Cite this Item
An Ankyrin-Related Gene (unc-44) Is Necessary for Proper Axonal Guidance in Caenorhabditis elegans
Preview not available

Abstract

Caenorhabditis elegans unc-44 mutations result in aberrant axon guidance and fasciculation with inappropriate partners. The unc-44 gene was cloned by transposon tagging, and verified by genetic and molecular analyses of six transposon-induced alleles and their revertants. Nucleotide sequence analyses demonstrated that unc-44 encodes a series of putative ankyrin-related proteins, including AO49 ankyrin (1815 aa, 198.8 kD), AO66 ankyrin (1867 aa, 204 kD), and AO13 ankyrin (≤4700 aa, ≤517 kD). In addition to the major set of ∼6 kb alternatively spliced transcripts, minor transcripts were observed at ∼3, 5, 7, and 14 kb. Evidence is provided that mutations in the ∼14-kb AO13 ankyrin transcript are responsible for the neuronal defects. These molecular studies provide the first evidence that ankyrin-related molecules are required for axonal guidance.

Page Thumbnails

  • Thumbnail: Page 
1081
    1081
  • Thumbnail: Page 
1082
    1082
  • Thumbnail: Page 
1083
    1083
  • Thumbnail: Page 
1084
    1084
  • Thumbnail: Page 
1085
    1085
  • Thumbnail: Page 
1086
    1086
  • Thumbnail: Page 
1087
    1087
  • Thumbnail: Page 
1088
    1088
  • Thumbnail: Page 
1089
    1089
  • Thumbnail: Page 
1090
    1090
  • Thumbnail: Page 
1091
    1091
  • Thumbnail: Page 
1092
    1092