Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Hepatocyte Growth Factor/Scatter Factor Modulates Cell Motility, Proliferation, and Proteoglycan Synthesis of Chondrocytes

Toshiaki Takebayashi, Masahiro Iwamoto, Akitoshi Jikko, Tomohiro Matsumura, Motomi Enomoto-Iwamoto, Fumio Myoukai, Eiki Koyama, Tomoichiro Yamaai, Kunio Matsumoto, Toshikazu Nakamura, Kojiro Kurisu and Sumihare Noji
The Journal of Cell Biology
Vol. 129, No. 5 (Jun., 1995), pp. 1411-1419
Stable URL: http://www.jstor.org/stable/1616966
Page Count: 9
  • More info
  • Cite this Item
Hepatocyte Growth Factor/Scatter Factor Modulates Cell Motility, Proliferation, and Proteoglycan Synthesis of Chondrocytes
Preview not available

Abstract

Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional growth factor that promotes proliferation, motility, and morphogenesis in epithelial cells. Recently the HGF receptor, c-met protooncogene product, has been shown to be expressed in developing limb buds (Sonnenberg, E., D. Meyer, M. Weidner, and C. Birchmeiyer. 1993. J. Cell Biol. 123:223-235), suggesting that some populations of mesenchymal cells in limb buds respond to HGF/SF. To test the possibility that HGF/SF is involved in regulation of cartilage development, we isolated chondrocytes from knee joints and costal cartilages of 23-d embryonic and 4-wk-old rabbits, and analyzed the effects of HGF/SF on migration and proliferation of these cells. We found that HGF/SF stimulated migration of cultured articular chondrocytes but did not scatter limb mesenchymal fibroblasts or synovial fibroblasts in culture. HGF/SF also stimulated proliferation of chondrocytes; a maximum three-fold stimulation in DNA synthesis was observed at the concentration of 3 ng/ml of HGF/SF. Moreover, HGF/SF had the ability to enhance proteoglycan synthesis in chondrocytes. The responsiveness of chondrocytes to HGF/SF was also supported by the observation that they expressed the HGF/SF receptor. Addition of the neutralizing antibody to rat HGF/SF affected neither DNA synthesis nor proteoglycan synthesis in rat chondrocytes, suggesting a paracine mechanism of action of HGF/SF on these cells. In situ hybridization analysis showed that HGF/SF mRNA was restrictively expressed in the areas of future joint regions in developing limb buds and in the intercostal spaces of developing costal cartilages. These findings suggest that HGF/SF plays important roles in cartilage development through its multiple activities.

Page Thumbnails

  • Thumbnail: Page 
1411
    1411
  • Thumbnail: Page 
1412
    1412
  • Thumbnail: Page 
1413
    1413
  • Thumbnail: Page 
1414
    1414
  • Thumbnail: Page 
1415
    1415
  • Thumbnail: Page 
1416
    1416
  • Thumbnail: Page 
1417
    1417
  • Thumbnail: Page 
1418
    1418
  • Thumbnail: Page 
1419
    1419