Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Interspecies Diversity of the Occludin Sequence: cDNA Cloning of Human, Mouse, Dog, and Rat-Kangaroo Homologues

Yuhko Ando-Akatsuka, Mitinori Saitou, Tetsuaki Hirase, Masashi Kishi, Akira Sakakibara, Masahiko Itoh, Shigenobu Yonemura, Mikio Furuse and Shoichiro Tsukita
The Journal of Cell Biology
Vol. 133, No. 1 (Apr., 1996), pp. 43-47
Stable URL: http://www.jstor.org/stable/1617516
Page Count: 5
  • More info
  • Cite this Item
Interspecies Diversity of the Occludin Sequence: cDNA Cloning of Human, Mouse, Dog, and Rat-Kangaroo Homologues
Preview not available

Abstract

Occludin has been identified from chick liver as a novel integral membrane protein localizing at tight junctions. To analyze and modulate the functions of tight junctions, it would be advantageous to know the mammalian homologues of occludin and their genes. Here we describe the nucleotide sequences of full length cDNAs encoding occludin of rat-kangaroo (potoroo), human, mouse, and dog. Rat-kangaroo occludin cDNA was prepared from RNA isolated from PtK2 cell culture, using a mAb against chicken occludin, whereas the others were amplified by polymerase chain reaction based on the sequence found around the human neuronal apoptosis inhibitory protein gene. The amino acid sequences of the three mammalian (human, murine, and canine) occludins were very closely related to each other (∼90% identity), whereas they diverged considerably from those of chicken and rat-kangaroo (∼50% identity). Implications of these data and novel experimental options in cell biological research are discussed.

Page Thumbnails

  • Thumbnail: Page 
43
    43
  • Thumbnail: Page 
44
    44
  • Thumbnail: Page 
45
    45
  • Thumbnail: Page 
46
    46
  • Thumbnail: Page 
47
    47