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emo-1, a Caenorhabditis elegans Sec61p γ Homologue, Is Required for Oocyte Development and Ovulation

Kouichi Iwasaki, James McCarter, Ross Francis and Tim Schedl
The Journal of Cell Biology
Vol. 134, No. 3 (Aug., 1996), pp. 699-714
Stable URL: http://www.jstor.org/stable/1617586
Page Count: 16
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emo-1, a Caenorhabditis elegans Sec61p γ Homologue, Is Required for Oocyte Development and Ovulation
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Abstract

emo-1(oz1) is a member of a class of hermaphrodite sterile mutations in Caenorhabditis elegans that produce endomitotic oocytes in the gonad arm. Oocytes in emo-1(oz1) mutants exhibit multiple defects during oogenesis. After meiotic maturation, ovulation fails, trapping oocytes in the gonad arm where they become endomitotic. emo-1 encodes a homologue of the Sec61p γ subunit, a protein necessary for translocation of secretory and transmembrane proteins into the endoplasmic reticulum of yeast and mammalian cells. A putative emo-1 null mutation, oz151, displays embryonic lethality. The oz1 sterile mutation is a transposable element insertion into the emo-1 3′ untranslated region that almost completely eliminates germline mRNA accumulation. Genetic mosaic analysis using the oz1 allele indicates that emo-1(+) expression in germ cells is required for fertility. The J67 monoclonal antibody, which recognizes an oocyte surface antigen, does not stain oz1 oocytes, a finding consistent with defective protein transport in the mutant. We propose that the emo-1 gene product acts in the transport of secreted and transmembrane proteins in C. elegans oocytes, and is necessary for both oogenesis and the coupling of ovulation with meiotic maturation.

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