Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Neurofascin Induces Neurites by Heterophilic Interactions with Axonal NrCAM while NrCAM Requires F11 on the Axonal Surface to Extend Neurites

Hansjürgen Volkmer, Roland Leuschner, Ute Zacharias and Fritz G. Rathjen
The Journal of Cell Biology
Vol. 135, No. 4 (Nov., 1996), pp. 1059-1069
Stable URL: http://www.jstor.org/stable/1617760
Page Count: 11
  • More info
  • Cite this Item
Neurofascin Induces Neurites by Heterophilic Interactions with Axonal NrCAM while NrCAM Requires F11 on the Axonal Surface to Extend Neurites
Preview not available

Abstract

Neurofascin and NrCAM are two axon-associated transmembrane glycoproteins belonging to the L1 subgroup of the Ig superfamily. In this study, we have analyzed the interaction of both proteins using neurite outgrowth and binding assays. A neurofascin-Fc chimera was found to stimulate the outgrowth of tectal cells when immobilized on an inert surface but not as a soluble form using polylysine as substrate. Antibody blocking experiments demonstrate that neurite extension on immobilized neurofascin is mediated by NrCAM on the axonal surface. Under the reverse experimental conditions where NrCAM induces neurite extension, F11, and not neurofascin, serves as axonal receptor. Binding studies using transfected COS7 cells and immunoprecipitations reveal a direct interaction between neurofascin and NrCAM. This binding activity was mapped to the Ig domains within neurofascin. The neurofascin-NrCAM binding can be modulated by alternative splicing of specific stretches within neuro-fascin. These studies indicate that heterophilic interactions between Ig-like proteins implicated in axonal extension underlie a regulation by the neuron.

Page Thumbnails

  • Thumbnail: Page 
1059
    1059
  • Thumbnail: Page 
1060
    1060
  • Thumbnail: Page 
1061
    1061
  • Thumbnail: Page 
1062
    1062
  • Thumbnail: Page 
1063
    1063
  • Thumbnail: Page 
1064
    1064
  • Thumbnail: Page 
1065
    1065
  • Thumbnail: Page 
1066
    1066
  • Thumbnail: Page 
1067
    1067
  • Thumbnail: Page 
1068
    1068
  • Thumbnail: Page 
1069
    1069