Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Calcineurin-GATA-6 Pathway Is Involved in Smooth Muscle-Specific Transcription

Hiromichi Wada, Koji Hasegawa, Tatsuya Morimoto, Tsuyoshi Kakita, Tetsuhiko Yanazume, Mitsuru Abe and Shigetake Sasayama
The Journal of Cell Biology
Vol. 156, No. 6 (Mar. 18, 2002), pp. 983-991
Stable URL: http://www.jstor.org/stable/1620979
Page Count: 9
  • More info
  • Cite this Item
Calcineurin-GATA-6 Pathway Is Involved in Smooth Muscle-Specific Transcription
Preview not available

Abstract

Intracellular calcium is one of the important signals that initiates the myogenic program. The calcium-activated phosphatase calcineurin is necessary for the nuclear import of the nuclear factor of activated T cell (NFAT) family members, which interact with zinc finger GATA transcription factors. Whereas GATA-6 plays a role in the maintenance of the differentiated phenotype in vascular smooth muscle cells (VSMCs), it is unknown whether the calcineurin pathway is associated with GATA-6 and plays a role in the differentiation of VSMCs. The smooth muscle-myosin heavy chain (Sm-MHC) gene is a downstream target of GATA-6, and provides a highly specific marker for differentiated VSMCs. Using immunoprecipitation Western blotting, we showed that NFATc1 interacted with GATA-6. Consistent with this, NFATc1 further potentiated GATA-6-activated Sm-MHC transcription. Induction of VSMCs to the quiescent phenotype caused nuclear translocation of NFATc1. In differentiated VSMCs, blockage of calcineurin down-regulated the amount of GATA-6-DNA binding as well as the expression of Sm-MHC and its transcriptional activity. These findings demonstrate that the calcineurin pathway is associated with GATA-6 and is required for the maintenance of the differentiated phenotype in VSMCs.

Page Thumbnails

  • Thumbnail: Page 
983
    983
  • Thumbnail: Page 
984
    984
  • Thumbnail: Page 
985
    985
  • Thumbnail: Page 
986
    986
  • Thumbnail: Page 
987
    987
  • Thumbnail: Page 
988
    988
  • Thumbnail: Page 
989
    989
  • Thumbnail: Page 
990
    990
  • Thumbnail: Page 
991
    991