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The Sec34/Sec35p Complex, a Ypt1p Effector Required for Retrograde Intra-Golgi Trafficking, Interacts with Golgi SNAREs and COPI Vesicle Coat Proteins

Elena S. Suvorova, Rainer Duden and Vladimir V. Lupashin
The Journal of Cell Biology
Vol. 157, No. 4 (May 13, 2002), pp. 631-643
Stable URL: http://www.jstor.org/stable/1621085
Page Count: 13
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The Sec34/Sec35p Complex, a Ypt1p Effector Required for Retrograde Intra-Golgi Trafficking, Interacts with Golgi SNAREs and COPI Vesicle Coat Proteins
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Abstract

The Sec34/35 complex was identified as one of the evolutionarily conserved protein complexes that regulates a cis-Golgi step in intracellular vesicular transport. We have identified three new proteins that associate with Sec35p and Sec34p in yeast cytosol. Mutations in these Sec34/35 complex subunits result in defects in basic Golgi functions, including glycosylation of secretory proteins, protein sorting, and retention of Golgi resident proteins. Furthermore, the Sec34/35 complex interacts genetically and physically with the Rab protein Ypt1p, intra-Golgi SNARE molecules, as well as with Golgi vesicle coat complex COPI. We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles.

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