Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

The Conditional Inactivation of the β-Catenin Gene in Endothelial Cells Causes a Defective Vascular Pattern and Increased Vascular Fragility

Anna Cattelino, Stefan Liebner, Radiosa Gallini, Adriana Zanetti, Giovanna Balconi, Alessandro Corsi, Paolo Bianco, Hartwig Wolburg, Robert Moore, Boussadia Oreda, Rolf Kemler and Elisabetta Dejana
The Journal of Cell Biology
Vol. 162, No. 6 (Sep. 15, 2003), pp. 1111-1122
Stable URL: http://www.jstor.org/stable/1621795
Page Count: 12
  • More info
  • Cite this Item
The Conditional Inactivation of the β-Catenin Gene in Endothelial Cells Causes a Defective Vascular Pattern and Increased Vascular Fragility
Preview not available

Abstract

Using the Cre/loxP system we conditionally inactivated β-catenin in endothelial cells. We found that early phases of vasculogenesis and angiogenesis were not affected in mutant embryos; however, vascular patterning in the head, vitelline, umbilical vessels, and the placenta was altered. In addition, in many regions, the vascular lumen was irregular with the formation of lacunae at bifurcations, vessels were frequently hemorrhagic, and fluid extravasation in the pericardial cavity was observed. Cultured β-catenin -/- endothelial cells showed a different organization of intercellular junctions with a decrease in α-catenin in favor of desmoplakin and marked changes in actin cytoskeleton. These changes paralleled a decrease in cell-cell adhesion strength and an increase in paracellular permeability. We conclude that in vivo, the absence of β-catenin significantly reduces the capacity of endothelial cells to maintain intercellular contacts. This may become more marked when the vessels are exposed to high or turbulent flow, such as at bifurcations or in the beating heart, leading to fluid leakage or hemorrhages.

Page Thumbnails

  • Thumbnail: Page 
1111
    1111
  • Thumbnail: Page 
1112
    1112
  • Thumbnail: Page 
1113
    1113
  • Thumbnail: Page 
1114
    1114
  • Thumbnail: Page 
1115
    1115
  • Thumbnail: Page 
1116
    1116
  • Thumbnail: Page 
1117
    1117
  • Thumbnail: Page 
1118
    1118
  • Thumbnail: Page 
1119
    1119
  • Thumbnail: Page 
1120
    1120
  • Thumbnail: Page 
1121
    1121
  • Thumbnail: Page 
1122
    1122