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Phagocytosis: Flow Cytometric Quantitation with Fluorescent Microspheres
John A. Steinkamp, Julie S. Wilson, George C. Saunders and Carleton C. Stewart
New Series, Vol. 215, No. 4528 (Jan. 1, 1982), pp. 64-66
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/1688539
Page Count: 3
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The phagocytosis of uniform fluorescent latex particles by pulmonary macrophages in the rat was analyzed by flow cytometric methods. The percentage of phagocytic macrophages and the number of particles per cell were determined from cell-size and fluorescence histograms. A comparison of in vivo and in vitro phagocytosis data showed that the percentage of phagocytic lavaged macrophages reflected the availability of instilled particles. With sodium azide used to model phagocytosis inhibition, it was shown that the percentage of phagocytic cells and the number of particles per cell can be determined simultaneously.
Science © 1982 American Association for the Advancement of Science