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Microholography of Living Organisms

Johndale C. Solem and George C. Baldwin
Science
New Series, Vol. 218, No. 4569 (Oct. 15, 1982), pp. 229-235
Stable URL: http://www.jstor.org/stable/1689231
Page Count: 7
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Microholography of Living Organisms
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Abstract

By using intense pulsed coherent x-ray sources that are currently under development, it will be possible to obtain magnified three-dimensional images of elementary biological structures in the living state at precisely defined instants. For optimum contrast, sensitivity, and resolution, the hologram should be made with x-rays tuned to a resonance of nitrogen near 0.3 nanometer. Resolution will then be limited mainly by the hydrodynamic expansion that occurs while the necessary number of photons is being registered. Problems of technique are also briefly discussed.

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