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Initiation of Endochondral Calcification is Related to Changes in the Redox State of Hypertrophic Chondrocytes

Irving M. Shapiro, Ellis E. Golub, Saburo Kakuta, John Hazelgrove, Julie Havery, Britton Chance and Peter Frasca
Science
Vol. 217, No. 4563 (Sep. 3, 1982), pp. 950-952
Stable URL: http://www.jstor.org/stable/1689356
Page Count: 3
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Initiation of Endochondral Calcification is Related to Changes in the Redox State of Hypertrophic Chondrocytes
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Abstract

The level of pyridine nucleotides (NADH and NAD$^{+}$) in the mineralizing growth plate of the chick was ascertained by high-resolution scanning microfluorimetry and biochemical analysis. Scanning electron microscopy and light microscopy were used to relate the concentrations of NADH and NAD$^{+}$ to stages of chondrocyte maturation. A dramatic increase was found in the relative concentration of reduced pyridine nucleotides in the hypertrophic zone. On either side of this zone, in proliferating and calcifying cartilage, there was a decrease in NADH fluorescence, and the NADH/NAD$^{+}$ ratio was depressed. The finding that NADH accumulated in the tissue zone associated with the earliest deposition of bone mineral supports the hypothesis that a change in the redox state initiates tissue mineralization.

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