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DNA Cloning of Plasmodium falciparum Circumsporozoite Gene: Amino Acid Sequence of Repetitive Epitope

Vincenzo Enea, Joan Ellis, Fidel Zavala, David E. Arnot, Achara Asavanich, Aoi Masuda, Isabella Quakyi and Ruth S. Nussenzweig
Science
New Series, Vol. 225, No. 4662 (Aug. 10, 1984), pp. 628-630
Stable URL: http://www.jstor.org/stable/1694045
Page Count: 3
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DNA Cloning of Plasmodium falciparum Circumsporozoite Gene: Amino Acid Sequence of Repetitive Epitope
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Abstract

A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence: proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS β-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.

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