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Cultivation of the Drosophila Sex-Ratio Spiroplasma

Kevin J. Hackett, Dwight E. Lynn, David L. Williamson, Annette S. Ginsberg and Robert F. Whitcomb
Science
New Series, Vol. 232, No. 4755 (Jun. 6, 1986), pp. 1253-1255
Stable URL: http://www.jstor.org/stable/1697140
Page Count: 3
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Cultivation of the Drosophila Sex-Ratio Spiroplasma
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Abstract

Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R$^{2}$). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms and facilitates study of the sex-ratio trait in Drosophila.

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