Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Upstream Operators Enhance Repression of the lac Promoter

Michael C. Mossing and M. Thomas Record
Science
New Series, Vol. 233, No. 4766 (Aug. 22, 1986), pp. 889-892
Stable URL: http://www.jstor.org/stable/1697232
Page Count: 4
  • More info
  • Cite this Item
Upstream Operators Enhance Repression of the lac Promoter
Preview not available

Abstract

To study regulation of transcription by distant elements, a wild-type lac operator was inserted upstream of a promoter-constitutive operator control region. The upstream operator is shown to aid in repression of transcription from the mutant control region. The effectiveness of the upstream operator as a function of its distance from the mutant control region parallels the length dependence observed for DNA cyclization. A quantitative model is proposed for action-at-a-distance of DNA control sites in which protein-protein and protein-DNA interactions are mediated by DNA looping. In this model, the effective concentrations of interacting proteins that are tethered by DNA are determined by the length of the intervening DNA and by its inherent bending and torsional stiffness. This model makes a number of predictions for both eukaryotic and prokaryotic control sequences located far from their sites of action.

Page Thumbnails

  • Thumbnail: Page 
889
    889
  • Thumbnail: Page 
890
    890
  • Thumbnail: Page 
891
    891
  • Thumbnail: Page 
892
    892