Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Human CSF-1: Molecular Cloning and Expression of 4-kb cDNA Encoding the Human Urinary Protein

Gordon G. Wong, Patricia A. Temple, Anne C. Leary, JoAnn S. Witek-Giannotti, Yu-Chung Yang, Agnes B. Ciarletta, Margaret Chung, Patricia Murtha, Ronald Kriz, Randal J. Kaufman, Catherine R. Ferenz, Barbara S. Sibley, Katherine J. Turner, Rodney M. Hewick, Steven C. Clark, Nobuya Yanai, Hajime Yokota, Muneo Yamada, Minoru Saito, Kazuo Motoyoshi and Fumimaro Takaku
Science
New Series, Vol. 235, No. 4795 (Mar. 20, 1987), pp. 1504-1508
Stable URL: http://www.jstor.org/stable/1699054
Page Count: 5
  • More info
  • Cite this Item
Human CSF-1: Molecular Cloning and Expression of 4-kb cDNA Encoding the Human Urinary Protein
Preview not available

Abstract

A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.

Page Thumbnails

  • Thumbnail: Page 
1504
    1504
  • Thumbnail: Page 
1505
    1505
  • Thumbnail: Page 
1506
    1506
  • Thumbnail: Page 
1507
    1507
  • Thumbnail: Page 
1508
    1508