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Human CSF-1: Molecular Cloning and Expression of 4-kb cDNA Encoding the Human Urinary Protein
Gordon G. Wong, Patricia A. Temple, Anne C. Leary, JoAnn S. Witek-Giannotti, Yu-Chung Yang, Agnes B. Ciarletta, Margaret Chung, Patricia Murtha, Ronald Kriz, Randal J. Kaufman, Catherine R. Ferenz, Barbara S. Sibley, Katherine J. Turner, Rodney M. Hewick, Steven C. Clark, Nobuya Yanai, Hajime Yokota, Muneo Yamada, Minoru Saito, Kazuo Motoyoshi and Fumimaro Takaku
New Series, Vol. 235, No. 4795 (Mar. 20, 1987), pp. 1504-1508
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/1699054
Page Count: 5
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A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.
Science © 1987 American Association for the Advancement of Science