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Kaposi's Sarcoma Cells: Long-Term Culture with Growth Factor from Retrovirus-Infected CD4$^{+}$ T Cells

Shuji Nakamura, S. Zaki Salahuddin, Peter Biberfeld, Barbara Ensoli, Phillip D. Markham, Flossie Wong-Staal and Robert C. Gallo
Science
New Series, Vol. 242, No. 4877 (Oct. 21, 1988), pp. 426-430
Stable URL: http://www.jstor.org/stable/1702601
Page Count: 5
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Kaposi's Sarcoma Cells: Long-Term Culture with Growth Factor from Retrovirus-Infected CD4$^{+}$ T Cells
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Abstract

Studies of the biology and pathogenesis of Kaposi's sarcoma (KS) have been hampered by the inability to maintain long-term cultures of KS cells in vitro. In this study AIDS-KS--derived cells with characteristic spindle-like morphology were cultured with a growth factor (or factors) released by CD4$^{+}$ T lymphocytes infected with human T-lymphotropic virus type I or II (HTLV-I or HTLV-II) or with human immunodeficiency virus type 1 or 2 (HIV-1 or HIV-2). Medium conditioned by HTLV-II--infected, transformed lines of T cells (HTLV-II CM) contained large amounts of this growth activity and also supported the temporary growth of normal vascular endothelial cells, but not fibroblasts. Interleukin-1 and tumor necrosis factor--$\alpha $ stimulated the growth of the KS-derived cells, but the growth was only transient and these factors could be distinguished from that in HTLV-II CM. Other known endothelial cell growth promoting factors, such as acidic and basic fibroblast growth factors and epidermal growth factor, did not support the long-term growth of the AIDS-KS cells. The factor released by CD4$^{+}$ T cells infected with human retroviruses should prove useful in studies of the pathogenesis of KS.

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