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Journal Article

Calcium-Activated Adenosine Triphosphatase Localization in Cultured Beating Heart Cells

Hazel Lewis and Isaac Harary
Science
New Series, Vol. 141, No. 3575 (Jul. 5, 1963), pp. 47-48
Stable URL: http://www.jstor.org/stable/1710902
Page Count: 2
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Abstract

The calcium-activated adenosine triphosphatase activity of cultured single beating heart cells of the rat was localized and visualized by incubating the cells, after extraction in a mixture of glycerol and water, with adenosine triphosphate and calcium. The calcium phosphate, precipitated at the site of the enzyme reaction, was converted into a visible precipitate. The enzyme activity appeared in bands perpendicular to the myofibrils.

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