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Culture of Insect Salivary Glands in a Chemically Defined Medium

Grace B. Cannon
New Series, Vol. 146, No. 3647 (Nov. 20, 1964), p. 1063
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A method for culturing the salivary glands of Sciara coprophila in a chemically defined medium has been developed. During a 24-hour period, the polytene chromosomes from six larval stages undergo normal developmental changes, including puff formation, puff condensation, and DNA synthesis.

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