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Satellite DNA in Constitutive Heterochromatin of the Guinea Pig
Jorge J. Yunis and Walid G. Yasmineh
New Series, Vol. 168, No. 3928 (Apr. 10, 1970), pp. 263-265
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/1728385
Page Count: 3
You can always find the topics here!Topics: DNA, Satellite DNA, Heterochromatin, Chromatin, Euchromatin, Liver, Centrifugation, Butchering, Cell nucleolus, Base composition
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Total DNA and DNA from the heterochromatin and euchromatin fractions of male guinea pig liver nuclei were analyzed by cesium sulfate-silver density-gradient centrifugation. Total DNA is composed of three components: a heavy satellite DNA, a main DNA of intermediate density, and a light satellite DNA. Heterochromatin DNA shows a fourfold enrichment in the satellite components while euchromatin DNA is relatively devoid of them. The strands of both satellite DNA's are separable by centrifugation in alkaline cesium chloride. Base analyses on the separate strands demonstrate that the two satellite DNA's represent different species.
Science © 1970 American Association for the Advancement of Science