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Satellite DNA in Constitutive Heterochromatin of the Guinea Pig

Jorge J. Yunis and Walid G. Yasmineh
Science
New Series, Vol. 168, No. 3928 (Apr. 10, 1970), pp. 263-265
Stable URL: http://www.jstor.org/stable/1728385
Page Count: 3
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Satellite DNA in Constitutive Heterochromatin of the Guinea Pig
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Abstract

Total DNA and DNA from the heterochromatin and euchromatin fractions of male guinea pig liver nuclei were analyzed by cesium sulfate-silver density-gradient centrifugation. Total DNA is composed of three components: a heavy satellite DNA, a main DNA of intermediate density, and a light satellite DNA. Heterochromatin DNA shows a fourfold enrichment in the satellite components while euchromatin DNA is relatively devoid of them. The strands of both satellite DNA's are separable by centrifugation in alkaline cesium chloride. Base analyses on the separate strands demonstrate that the two satellite DNA's represent different species.

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