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Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

Richard C. Weisenberg
Science
New Series, Vol. 177, No. 4054 (Sep. 22, 1972), pp. 1104-1105
Stable URL: http://www.jstor.org/stable/1734230
Page Count: 2
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Abstract

Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.

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