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An Isomorphous Heavy-Atom Derivative of Crystalline Formylmethionine Transfer RNA
R. W. Schevitz, M. A. Navia, D. A. Bantz, G. Cornick, J. J. Rosa, M. D. H. Rosa and P. B. Sigler
New Series, Vol. 177, No. 4047 (Aug. 4, 1972), pp. 429-431
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/1734717
Page Count: 3
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An isomorphous osmium derivative of crystalline yeast initiator transfer RNA has been prepared and interpreted to 6-angstrom resolution. The coordinates of the heavy atoms have been determined by Patterson and "direct" methods applied to the difference coefficients of the centric projections, followed by least-squares refinement. There is one dominant site per asymmetric unit, consistent with the finding by neutron-activation analysis that there is approximately one osmium atom per molecule of transfer RNA. The osmium derivative appears to be a normal substrate for enzymatic aminoacylation.
Science © 1972 American Association for the Advancement of Science