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Production of Cocoa Plants (Theobroma cacao L.) via Micrografting of Somatic Embryos
Maria E. Aguilar, Victor M. Villalobos and Nelly Vasquez
In Vitro Cellular & Developmental Biology. Plant
Vol. 28P, No. 1 (Jan., 1992), pp. 15-19
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/20064805
Page Count: 5
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The possibilities for in vitro regeneration of cocoa plants have been limited. Somatic embryos can differentiate from cotyledonary cells, but their conversion into plants has remained largely unsolved. In the present study, we attempted micrografting of somatic embryos to seedling rootstocks. Different conditions were analyzed for the rootstocks and embryos, and it was found that complete plant regeneration eeeded about 10 mo. The best results were obtained using a simple culture medium, 3-wk-old rootstocks, and somatic embryos without cotyledons. Histologic events associated with the graft union were also analyzed. Cells, mainly from the rootstock, initiate cellular division in different patterns, producing a callus at the graft junction. Within this region some cells differentiated into zylem and phloem, establishing vascular connection in the graft. Afterward, micrografted plants start to grow and differentiate new leaves and roots allowing for transfer to soil.
In Vitro Cellular & Developmental Biology. Plant © 1992 Society for In Vitro Biology