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Aeration: A Simple Method to Control Vitrification and Improve in vitro Culture of Rare Australian Plants

Maurizio Rossetto, Kingsley W. Dixon and Eric Bunn
In Vitro Cellular & Developmental Biology. Plant
Vol. 28P, No. 4 (Oct., 1992), pp. 192-196
Stable URL: http://www.jstor.org/stable/20064846
Page Count: 5
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Aeration: A Simple Method to Control Vitrification and Improve in vitro Culture of Rare Australian Plants
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Abstract

Aeration of tissue cultured rare Australian plants Conostylis wonganensis S. D. Hopper (Haemodoraceae); Diplolaena andrewsii Ostenf.; Drummondita ericoides Harvey (Rutaceae); Eremophila resinosa F. Muell. (Myoporaceae); Eucalyptus 'graniticola' (Myrtaceae); Lechenaultia pulvinaris C. Gardner (Goodeniaceae); and Sowerbaea multicaulis E. Pritzel (Liliaceae) has been found to reduce vitrification in sensitive species as well as significantly improving shoot quality and transfer to soil in most study species. A simple 7-mm hole with a double-layer insert of filter paper in the polypropylene screw lids of the culture vessel decreased shoot vitrification over a 4-wk culture period. The method has implications for facilitating the tissue culture of other rare Australian plants and reducing the occurrence of this developmental abnormality.

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