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Micropropagation of Hesperaloe parviflora
Arthur M. Richwine, Jimmy L. Tipton and Gary A. Thompson
In Vitro Cellular & Developmental Biology. Plant
Vol. 32, No. 4 (Oct. - Dec., 1996), pp. 262-266
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/20064920
Page Count: 5
You can always find the topics here!Topics: Plants, Pedicels, Flower buds, Inflorescences, Micropropagation, Cytokinins, Plant cells, Diameters, Mature plants, Rhizomes
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We successfully micropropagated Hesperaloe parviflora from mature plants. Shoot cultures were directly initiated from mature plants using pedicel bud explants on a modified Murashige and Skoog medium containing Nitsch and Nitsch vitamins and 1 μM zeatin riboside. Axillary shoot multiplication from established cultures was most responsive to changing concentrations of N⁶-benzyladenine (BA) with the greatest production on 6 μM BA. Growing shoots on a medium supplemented with 6 μM BA for 6 wk and then transferring cultures to a 1 μM BA medium for 6 more wk increased the number of transferable shoots, but not significantly. However, our data predicts that the maximum number of transferable shoots produced from a single microshoot would occur on media with 5.4 μM zeatin riboside. Shoots rooted easily in vitro or ex vitro and rooted shoots were easily acclimatized. The methods described in this paper are being used to commercially micropropagate H. parviflora.
In Vitro Cellular & Developmental Biology. Plant © 1996 Society for In Vitro Biology