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Cryopreservation and Long-Term Storage of Pear Germplasm
Barbara M. Reed, Jeanine Denoma, Jie Luo, Yongjian Chang and Leigh Towill
In Vitro Cellular & Developmental Biology. Plant
Vol. 34, No. 3 (Jul. - Sep., 1998), pp. 256-260
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/20064993
Page Count: 5
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Germplasm collections of vegetatively propagated crops are usually maintained as plants in fields or potted in greenhouses or screened enclosures. Safety duplication of these collections, as duplicate plants or separate collections, is costly and requires large amounts of space. Cryopreservation techniques which were recently developed for long-term storage of pear germplasm may offer an efficient alternative to conventional germplasm collection maintenance. Pear (Pyrus L.) germplasm may now be stored as seeds (species), dormant buds or pollen from field-grown trees, or shoot tips from in vitro-grown plants (cultivars). Pear germplasm may now be cryopreserved and stored for long periods (> 100 yr) utilizing slow-freezing or vitrification of in vitro-grown shoot-tips. Dormant bud freezing, pollen, and seed cryopreservation of other lines are being developed to complete the base collection for Pyrus. This cryopreserved collection provides base (long-term) storage for the field-grown pear germplasm collection at the National Clonal Germplasm Repository, Corvallis, Oregon.
In Vitro Cellular & Developmental Biology. Plant © 1998 Society for In Vitro Biology