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T-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Asparatate Residue

Nico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini and Arp Schnittger
The Plant Cell
Vol. 19, No. 3 (Mar., 2007), pp. 972-985
Stable URL: http://www.jstor.org/stable/20076989
Page Count: 14
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T-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Asparatate Residue
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Abstract

As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases. Phosphorylation of a canonical Thr residue in the T-loop of the kinases is required for high enzyme activity in animals and yeast. We show that the Arabidopsis thaliana $\text{Cdc}2^{+}/\text{Cdc}28$ homolog CDKA;1 is also phosphorylated in the T-loop and that phosphorylation at the conserved Thr-161 residue is essential for its function. A phospho-mimicry T161D substitution restored the primary defect of cdka;1 mutants, and although the T161D substitution displayed a dramatically reduced kinase activity with a compromised ability to bind substrates, homozygous mutant plants were recovered. The rescue by the T161D substitution, however, was not complete, and the resulting plants displayed various developmental abnormalities. For instance, even though flowers were formed, these plants were completely sterile as a result of a failure of the meiotic program, indicating that different requirements for CDKA;1 function are needed during plant development.

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