Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

The Nuclear-Encoded Factor HCF173 Is Involved in the Initiation of Translation of the psbA mRNA in Arabidopsis thaliana

Kerstin Schult, Karin Meierhoff, Susanne Paradies, Thomas Töller, Petra Wolff and Peter Westhoff
The Plant Cell
Vol. 19, No. 4 (Apr., 2007), pp. 1329-1346
Stable URL: http://www.jstor.org/stable/20077018
Page Count: 18
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The Nuclear-Encoded Factor HCF173 Is Involved in the Initiation of Translation of the psbA mRNA in Arabidopsis thaliana
Preview not available

Abstract

To gain insight into the biogenesis of photosystem II (PSII) and to identify auxiliary factors required for this process, we characterized the mutant hcf173 of Arabidopsis thaliana. The mutant shows a high chlorophyll fluorescence phenotype (hcf) and is severely affected in the accumulation of PSII subunits. In vivo labeling experiments revealed a drastically decreased synthesis of the reaction center protein D1. Polysome association experiments suggest that this is primarily caused by reduced translation initiation of the corresponding psbA mRNA. Comparison of mRNA steady state levels indicated that the psbA mRNA is significantly reduced in hcf173. Furthermore, the determination of the psbA mRNA half-life revealed an impaired RNA stability. The HCF173 gene was identified by map-based cloning, and its identity was confirmed by complementation of the hcf phenotype. HCF173 encodes a protein with weak similarities to the superfamily of the short-chain dehydrogenases/reductases. The protein HCF173 is localized in the chloroplast, where it is mainly associated with the membrane system and is part of a higher molecular weight complex. Affinity chromatography of an HCF173 fusion protein uncovered the psbA mRNA as a component of this complex.

Page Thumbnails

  • Thumbnail: Page 
[1329]
    [1329]
  • Thumbnail: Page 
1330
    1330
  • Thumbnail: Page 
1331
    1331
  • Thumbnail: Page 
1332
    1332
  • Thumbnail: Page 
1333
    1333
  • Thumbnail: Page 
1334
    1334
  • Thumbnail: Page 
1335
    1335
  • Thumbnail: Page 
1336
    1336
  • Thumbnail: Page 
1337
    1337
  • Thumbnail: Page 
1338
    1338
  • Thumbnail: Page 
1339
    1339
  • Thumbnail: Page 
1340
    1340
  • Thumbnail: Page 
1341
    1341
  • Thumbnail: Page 
1342
    1342
  • Thumbnail: Page 
1343
    1343
  • Thumbnail: Page 
1344
    1344
  • Thumbnail: Page 
1345
    1345
  • Thumbnail: Page 
1346
    1346