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Birth of a Siberian Tiger Cub (Panthera tigris altaica) following Laparoscopic Intrauterine Artificial Insemination

Ann M. Donoghue, Leslie A. Johnston, Douglas L. Armstrong, Lee G. Simmons and David E. Wildt
Journal of Zoo and Wildlife Medicine
Vol. 24, No. 2 (Jun., 1993), pp. 185-189
Stable URL: http://www.jstor.org/stable/20095262
Page Count: 5
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Birth of a Siberian Tiger Cub (Panthera tigris altaica) following Laparoscopic Intrauterine Artificial Insemination
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Abstract

A 7-yr-old female Siberian tiger (Panthera tigris altaica) was treated with equine chorionic gonadotropin followed 80 hr later by human chorionic gonadotropin (hCG) to stimulate follicular development and ovulation, respectively. Laparoscopy 46 hr after hCG revealed that the ovaries contained eight fresh corpora lutea and six preovulatory follicles. A 16-gauge catheter was inserted transabdominally into each uterine horn, and 16.8 × 10⁶ motile spermatozoa (from a male designated by the Siberian Tiger Species Survival Plan [SSP]) were inseminated. Pregnancy was confirmed 45 days after insemination by ultrasound, and a single healthy male cub was born at 111 days of gestation. These results demonstrate for the first time that assisted reproductive technology can be used to produce genetically valuable offspring from parents identified and recommended by an SSP program.

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