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Human-Specific Gain of Function in a Developmental Enhancer
Shyam Prabhakar, Axel Visel, Jennifer A. Akiyama, Malak Shoukry, Keith D. Lewis, Amy Holt, Ingrid Plajzer-Frick, Harris Morrison, David R. FitzPatrick, Veena Afzal, Len A. Pennacchio, Edward M. Rubin and James P. Noonan
New Series, Vol. 321, No. 5894 (Sep. 5, 2008), pp. 1346-1350
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/20144754
Page Count: 5
You can always find the topics here!Topics: Humans, Embryos, Chimpanzees, Evolution, Genomes, Limb buds, Transgenic animals, Reporter genes, Mathematical expressions, Vertebrates
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Changes in gene regulation are thought to have contributed to the evolution of human development. However, in vivo evidence for uniquely human developmental regulatory function has remained elusive. In transgenic mice, a conserved noncoding sequence (HACNS1) that evolved extremely rapidly in humans acted as an enhancer of gene expression that has gained a strong limb expression domain relative to the orthologous elements from chimpanzee and rhesus macaque. This gain of function was consistent across two developmental stages in the mouse and included the presumptive anterior wrist and proximal thumb. In vivo analyses with synthetic enhancers, in which human-specific substitutions were introduced into the chimpanzee enhancer sequence or reverted in the human enhancer to the ancestral state, indicated that 13 substitutions clustered in an 81--base pair module otherwise highly constrained among terrestrial vertebrates were sufficient to confer the human-specific limb expression domain.
Science © 2008 American Association for the Advancement of Science