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The Different Roles of Serum and Calcium in the Control of Proliferation of BALB/c 3T3 Mouse Cells

A. L. Boynton, J. F. Whitfield and R. J. Isaacs
In Vitro
Vol. 12, No. 2 (Feb., 1976), pp. 120-123
Stable URL: http://www.jstor.org/stable/20170273
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The Different Roles of Serum and Calcium in the Control of Proliferation of BALB/c 3T3 Mouse Cells
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Abstract

Proliferatively inactive BALB/c 3T3 mouse cells in dense cultures initiate a growth-division cycle upon exposure to fresh calf serum in a low-calcium (0.01 mM) medium. If these calcium-deprived cells are not supplied with calcium sometime during the first 10 hours after serum stimulation, they will rapidly return to a proliferatively inactive state without initiating DNA synthesis. The prereplicative development of such stimulated calcium-deprived cells appears to stop at an advanced stage, because addition of calcium as late as 10 hours after serum exposure rapidly initiates DNA synthesis, and enables the culture's DNA-synthetic activity subsequently to reach its peak value at the same time as in control cultures.

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