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Rat Serum Stimulates Serotonin N-Acetyltransferase Activity in Pineal Monolayer Cultures
James Parr and Vernon D. Rowe
Vol. 18, No. 4 (Apr., 1982), pp. 361-368
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/20170454
Page Count: 8
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The effect of rat serum on serotonin N-acetyltransferase (NAT) activity and indole synthesis in monolayer cultures of neonatal rat pineal glands was examined. The addition of 5% rat serum to these cultures resulted in stimulation of NAT activity equal to that obtained with optimal concentrations of the adrenergic agonist norepinephrine (NE). Rat serum also increased the synthesis of both N-acetylserotonin and melatonin from tryptophan. Stimulation of NAT activity by rat serum was partially blocked by metoprolol and propranolol, but not by phentolamine or butoxamine. The serum factor responsible for the stimulation was stable to both freezing and boiling. No significant amounts of epinephrine, norepinephrine, or dopamine were detected in the serum.
In Vitro © 1982 Society for In Vitro Biology