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Rat Serum Stimulates Serotonin N-Acetyltransferase Activity in Pineal Monolayer Cultures

James Parr and Vernon D. Rowe
In Vitro
Vol. 18, No. 4 (Apr., 1982), pp. 361-368
Stable URL: http://www.jstor.org/stable/20170454
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Rat Serum Stimulates Serotonin N-Acetyltransferase Activity in Pineal Monolayer Cultures
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Abstract

The effect of rat serum on serotonin N-acetyltransferase (NAT) activity and indole synthesis in monolayer cultures of neonatal rat pineal glands was examined. The addition of 5% rat serum to these cultures resulted in stimulation of NAT activity equal to that obtained with optimal concentrations of the adrenergic agonist norepinephrine (NE). Rat serum also increased the synthesis of both N-acetylserotonin and melatonin from tryptophan. Stimulation of NAT activity by rat serum was partially blocked by metoprolol and propranolol, but not by phentolamine or butoxamine. The serum factor responsible for the stimulation was stable to both freezing and boiling. No significant amounts of epinephrine, norepinephrine, or dopamine were detected in the serum.

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