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Visualization of Growth Signal Transduction Cascades in Living Cells with Genetically Encoded Probes Based on Förster Resonance Energy Transfer
Kazuhiro Aoki, Etsuko Kiyokawa, Takeshi Nakamura and Michiyuki Matsuda
Philosophical Transactions: Biological Sciences
Vol. 363, No. 1500, Japan: Its Tradition and Hot Topics in Biological Sciences (Jun. 27, 2008), pp. 2143-2151
Published by: Royal Society
Stable URL: http://www.jstor.org/stable/20208623
Page Count: 9
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Fluorescence probes based on the principle of Förster resonance energy transfer (FRET) have shed new light on our understanding of signal transduction cascades. Among them, unimolecular FRET probes containing fluorescence proteins are rapidly increasing in number because these genetically encoded probes can be easily loaded into living cells and allow simple acquisition of FRET images. We have developed probes for small GTPases, tyrosine kinases, serine-threonine kinases and phosphoinositides. Images obtained with these probes have revealed that membrane protrusions such as nascent lamellipodia or neurites provide an active signalling platform in the growth factor-stimulated cells.
Philosophical Transactions: Biological Sciences © 2008 Royal Society