Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Visualization of Growth Signal Transduction Cascades in Living Cells with Genetically Encoded Probes Based on Förster Resonance Energy Transfer

Kazuhiro Aoki, Etsuko Kiyokawa, Takeshi Nakamura and Michiyuki Matsuda
Philosophical Transactions: Biological Sciences
Vol. 363, No. 1500, Japan: Its Tradition and Hot Topics in Biological Sciences (Jun. 27, 2008), pp. 2143-2151
Published by: Royal Society
Stable URL: http://www.jstor.org/stable/20208623
Page Count: 9
  • Read Online (Free)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Visualization of Growth Signal Transduction Cascades in Living Cells with Genetically Encoded Probes Based on Förster Resonance Energy Transfer
Preview not available

Abstract

Fluorescence probes based on the principle of Förster resonance energy transfer (FRET) have shed new light on our understanding of signal transduction cascades. Among them, unimolecular FRET probes containing fluorescence proteins are rapidly increasing in number because these genetically encoded probes can be easily loaded into living cells and allow simple acquisition of FRET images. We have developed probes for small GTPases, tyrosine kinases, serine-threonine kinases and phosphoinositides. Images obtained with these probes have revealed that membrane protrusions such as nascent lamellipodia or neurites provide an active signalling platform in the growth factor-stimulated cells.

Page Thumbnails

  • Thumbnail: Page 
2143
    2143
  • Thumbnail: Page 
2144
    2144
  • Thumbnail: Page 
2145
    2145
  • Thumbnail: Page 
2146
    2146
  • Thumbnail: Page 
2147
    2147
  • Thumbnail: Page 
2148
    2148
  • Thumbnail: Page 
2149
    2149
  • Thumbnail: Page 
2150
    2150
  • Thumbnail: Page 
2151
    2151