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In Vitro Selection and Regeneration of Chrysanthemum (Dendranthema grandiflorum Tzelev) Plants Resistant to Culture Filtrate of Septoria obesa Syd
S. Kumar, Sunil Kumar, S. P. Negi and J. K. Kanwar
In Vitro Cellular & Developmental Biology. Plant
Vol. 44, No. 6 (Nov. - Dec., 2008), pp. 474-479
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/20461749
Page Count: 6
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Callus cultures derived from leaf segments of chrysanthemum cultivar 'Snow Ball' which was susceptible to Septoria obesa were successfully used for in vitro selection for resistance to this pathogenic fungus. Resistant cell lines were selected by culturing callus on growth medium containing various concentrations of S. obesa filtrate. Resistant calluses obtained after two cycles (30 d each cycle) of selection were used for plant regeneration. About 30% of the plants regenerated from the resistant calluses and 70-80% of the plants raised from cuttings had acquired considerable resistance against the pathogen in the field. No phenotypic variation was observed in the selected regenerates.
In Vitro Cellular & Developmental Biology. Plant © 2008 Society for In Vitro Biology