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AN ARTIFICIAL LARVAL DIET FOR REARING OF ANASTREPHA STRIATA (DIPTERA: TEPHRITIDAE)

Emilio Hernández, J. Pedro Rivera, Dina Orozco-Davila, Miguel Salvador and Jorge Toledo
The Florida Entomologist
Vol. 93, No. 2 (June, 2010), pp. 167-174
Stable URL: http://www.jstor.org/stable/20720067
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
AN ARTIFICIAL LARVAL DIET FOR REARING OF ANASTREPHA STRIATA (DIPTERA: TEPHRITIDAE)
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Abstract

An artificial larval diet for Anastrepha striata (Schiner) was developed and the changes in the rearing and quality parameters through 6 generations during the adaptation were characterized. In the first experiment we tested diet formulations that had already been developed for the mass-rearing of Anastrepha ludens (Loew), A. obliqua (Macquart), A. serpentina (Wiedemann) and Ceratitis capitata (Wiedemann) by sowing A. striata eggs (20-40% hatched) in each diet. In those tested diets, the maximum larval recovery percentage was 4.82%. In the second experiment, in the AOII modified diet of A. obliqua, we substituted the protein source, torula yeast by Nutrifly™, torula yeast-casein and hydrolyzed protein. A formulated diet contained 4.83% Nutrifly™, 15% corn cob fractions, 8.0% corn flour, 8.33% sugar, 0.23% sodium benzoate, 0.11% nipagin, 0.13% citric acid, and 63.37% water allowed higher larval survival compared to diets with different protein sources. In the third experiment, we evaluated adaptation of the larvae to Nutrifly diet. Over 6 generations, the larval and pupal weights and pupation percentage decreased from parental to first generation and increased after the third generation, recovering the initial value. Larval recovery and adult emergence increased from parental generation to the next generations; and was maintained during the next 5 generations. Larval recovery only a light decreased in the third generation. The laboratory colonization of A. striata reared on this artificial diet required at least 5 generations for the larvae to adapt to the artificial diet and increase pupal weight and adult emergence. Se desarrollo una dieta para Anastrepha striata (Schiner), caracterizando los cambios ocurridos en los parámetros de cría y calidad durante seis generaciones. En el primer experimento se utilizaron huevos que registraron entre 20 y 40% de eclosión al momento de sembrarse sobre las dietas utilizadas en las crías de Anastrepha ludens (Loew), A. obliqua (Macquart), A. serpentina (Wiedemann) y Ceratitis capitata (Wiedemann). En estas dietas, la mayor recuperación larvaria fue de 4.8%. En el segundo experimento, en la dieta AOII modificada de A. obliqua fue sustituida la fuente de proteína, y la levadura torula por Nutrifly™, torula-caseína y proteína hidrolizada. En la dieta formulada con 4.83% de Nutrifly™, 15% de polvo de olote, 8.0% de harina de maíz, 8.33% de azúcar, 0.23% de benzoato de sodio, 0.11% de nipagín, 0.13% de ácido cítrico y 63.37% de agua, se obtuvo la mayor recuperación de larvas en comparación con las otras dietas formuladas con diferentes fuentes de proteína. En el tercer experimento, se caracterizó la adaptación de las larvas a la dieta Nutrifly™. Durante seis generaciones, el peso de larva y de pupa disminuyó de la generación parental a la primera generación, y ambos parámetros se incrementaron a partir de la tercera generación, hasta registrar un peso similar al observado en la generación progenitora. La recuperación larvaria y la emergencia de adultos se incrementó de la generación parental a las siguientes generaciones y mantuvo dicha tendencia durante las cinco generaciones, y solamente la recuperación larvaria registró una ligera disminución durante la tercera generación. Se discuten los resultados que la adaptación de Anastrepha striata a una dieta larvaria artificial requiere de al menos cinco generaciones.

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