Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Perturbation of lac Operator DNA Modification by Tryptic Core Protein from lac Repressor

Susan P. Manly, George N. Bennett and Kathleen Shive Matthews
Proceedings of the National Academy of Sciences of the United States of America
Vol. 80, No. 20, [Part 1: Biological Sciences] (Oct. 15, 1983), pp. 6219-6223
Stable URL: http://www.jstor.org/stable/23030
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Perturbation of lac Operator DNA Modification by Tryptic Core Protein from lac Repressor
Preview not available

Abstract

Operator DNA fragments were modified in the presence of lac repressor protein or its trypsin-resistant core. Operator DNA was alkylated or cleaved enzymatically with these related proteins present to compare the influences of their binding on the reactivities or enzymatic susceptibilities of individual bases in the sequence. These two protein species have pronounced and distinguishable effects on the reactivity of the bases of the operator fragment toward methylation by dimethyl sulfate. Perturbation of base alkylation by the trypsin-resistant core repressor is most pronounced in the inner, asymmetric region of the operator DNA, while repressor effects extend further on either end of the operator sequence. Digestion of the two protein-operator complexes by DNase I yields fragment patterns that differ primarily in extent of protection. These data extend the experimental base supporting the involvement of the core region of the lac repressor in addition to its NH2 termini in the operator-specific binding activity of this protein.

Page Thumbnails

  • Thumbnail: Page 
6219
    6219
  • Thumbnail: Page 
6220
    6220
  • Thumbnail: Page 
6221
    6221
  • Thumbnail: Page 
6222
    6222
  • Thumbnail: Page 
6223
    6223