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Reconstitution of a Functional Synaptosomal Membrane Possessing the Protein Constituents Involved in Acetylcholine Translocation

M. Israel, B. Lesbats, N. Morel, R. Manaranche, T. Gulik-Krzywicki and J. C. Dedieu
Proceedings of the National Academy of Sciences of the United States of America
Vol. 81, No. 1, [Part 1: Biological Sciences] (Jan. 1, 1984), pp. 277-281
Stable URL: http://www.jstor.org/stable/23205
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Reconstitution of a Functional Synaptosomal Membrane Possessing the Protein Constituents Involved in Acetylcholine Translocation
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Abstract

Reconstitution of a functional presynaptic membrane possessing calcium-dependent acetylcholine release properties has been achieved. The proteoliposomal membrane obtained gains its acetylcholine-releasing capabilities from presynaptic membrane proteins. At the peak of acetylcholine release, intramembrane particles became more numerous in one of the proteoliposomal membrane faces. This phenomenon resembles the intramembrane particle rearrangements found in stimulated synaptosomes. No visible structures capable of releasing acetylcholine as a result of the calcium influx were found inside the proteoliposomes. This supports the view that the release of free cytosolic acetylcholine from stimulated nerve terminals can be directly attributed to presynaptic membrane proteins. These proteins were extracted in a functional form from the synaptosomal membrane.

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