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Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro

Jinhong Shi, Haoming Li, Guohua Jin, Peipei Zhu, Meiling Tian, Jianbing Qin, Xuefeng Tan, Shuqing Zhao, Fuyu Wang, Yurong Hua and Yong Xiao
In Vitro Cellular & Developmental Biology. Animal
Vol. 48, No. 10 (November-December 2012), pp. 603-609
Stable URL: http://www.jstor.org/stable/23353392
Page Count: 7
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Abstract

Lhx8, also named L3, is a recently identified member of the LIM homeobox gene family. Previously, we found acetylcholinesterase (AChE)-positive cells in fimbria—fornix (FF) transected rat hippocampal subgranular zone (SGZ). In the present study, we detected choline acetyltransferase (ChAT)-positive cholinergic cells in hippocampal SGZ after FF transaction, and these ChAT-positive cells were double labeled by Lhx8. Then we overexpressed Lhx8 during neural differentiation of hippocampal neural stem/progenitor cells on adherent conditions using lentivirus Lenti6.3-Lhx8. The result indicated that overexpression of Lhx8 did not affect the proportion of MAP2-positive neurons, but increased the proportion of ChAT-positive cells in vitro. These results suggested that FF-transected hippocampal niche promoted the ChAT/Lhx8-positive cholinergic neurons generation in rodent hippocampus, and Lhx8 was not associated with the MAP2-positive neurons differentiation on adherent conditions, but played a role in the specification of cholinergic neurons derived from hippocampal neural stem/progenitor cells in vitro.

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