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Untersuchungen über die Bindung pflanzlicher Wuchsstoffe an verschiedene Komponenten des Chromatins in vitro: II. Versuche mit verschiedenen künstlich rekonstituierten Nucleoproteiden

Günter Fellenberg
Planta
Vol. 100, No. 4 (1971), pp. 347-356
Published by: Springer
Stable URL: http://www.jstor.org/stable/23369338
Page Count: 10
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Untersuchungen über die Bindung pflanzlicher Wuchsstoffe an verschiedene Komponenten des Chromatins in vitro: II. Versuche mit verschiedenen künstlich rekonstituierten Nucleoproteiden
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Abstract

Several growth substances (IAA, α-NAA) are able to reduce thermal stability of artificially reconstituted nucleoproteins without splitting off measurable amounts of protein from DNA. This effect is not shown by substances structurally related to auxins (β-NAA, tryptophan), but other growth substances (GA, KI) also reduce thermal stability of several reconstituated nucleoproteins. The effect of growth substances on the Tm of nucleoproteins strongly depends upon the concentration of the growth substances. The effective concentrations of IAA are lowered by increasing acidity of the protein component in the nucleoprotein. IAA and GA diminish the binding capacity of histones and residual nucleoproteins to DNA at different concentrations. Nucleoproteins containing histones and residual nuclear proteins (DNA/resid. prot. 1:0,5: 0,5) exhibited different thermal stability depending on whether part of the histones or residual nuclear proteins were first bound to DNA. Furthermore, these nucleoproteins showed different thermal stability after treatment with growth substances.

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