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In-vitro Binding of Phytochrome to a Particulate Fraction: A Function of Light Dose and Steady-state P fr Level
Peter H. Quail
Vol. 118, No. 4 (1974), pp. 357-360
Published by: Springer
Stable URL: http://www.jstor.org/stable/23371046
Page Count: 4
You can always find the topics here!Topics: Irradiation, Corn, Hypocotyls, Seedlings, Wavelengths, Particulate matter, Chromium, Irradiance, Coleoptiles, Molecules
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The binding of phytochrome to a particulate fraction in extracts from hypocotyl hooks of etiolated Cucurbita pepo L. seedlings has been examined as a function of the light dose and Pfr level established in vitro. As the steady-state level of Pfr transiently established in the 500 × g supernatant is increased, so the level of Pr subsequently pelletable at 20000 × g increases up to a saturation level. Increasing both the time and irradiance parameters of the light dose while holding the steady-state Pfr level constant, results similarly in increasing Pr pelletability. This agrees with results obtained previously with in-vivo irradiations of maize coleoptiles. Thus, like the in-vivo response, phytochrome binding in vitro appears to be a function of the total number of molecules converted to the Pfr form during the irradiation period.
Planta © 1974 Springer