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Release of Hydrolytic Enzymes from the Cell Walls of Intact and Disrupted Carrot Callus Tissue

D. R. Parr and J. Edelman
Planta
Vol. 127, No. 2 (1975), pp. 111-119
Published by: Springer
Stable URL: http://www.jstor.org/stable/23371867
Page Count: 9
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Release of Hydrolytic Enzymes from the Cell Walls of Intact and Disrupted Carrot Callus Tissue
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Abstract

The effect of ionic strength and pH on the solubility of acid invertase (E.C. 3.2.1.26.) and α-glucosidase (E.C. 3.2.1.20.) from carrot Daucus carota L. tissue cultures is presented. The action of these two factors on disrupted tissue is contrasted with the effects of the same treatment on the solubility of these enzymes from intact tissue. The results are discussed in terms of a unified theory on the solubility behaviour of acid invertase and other hydrolytic enzymes in plant cells.

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