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Hydroxyurea-induced Mitotic Synchronization in Allium sativum Root Meristems

Elisabeth Clain and Annie Brulfert
Planta
Vol. 150, No. 1 (1980), pp. 26-31
Published by: Springer
Stable URL: http://www.jstor.org/stable/23374901
Page Count: 6
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Hydroxyurea-induced Mitotic Synchronization in Allium sativum Root Meristems
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Abstract

The synchronized divisions following a treatment with hydroxyurea (HU) — an inhibitor of DNA synthesis — were studied in root meristems of Allium sativum using two methods: autoradiography of median sections and morphological labeling with a cytokinesis inhibitor. It is shown that the second wave of mitoses is heterogeneous: it is composed mostly of cells which have been synchronized in the S phase by the HU treatment, of cells coming from the quiescent center stimulated to enter DNA synthesis and of cells which were not blocked by the 23 h HU treatment (slow cycling cells). It is also shown that the cell cycle following the first synchronized division is considerably shortened by the synchronization procedure.

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